Table 3.
Stimulants used in the β-hexosaminidase degranulation assay (HDA).
| Stimulant | Concentration | Incubation time | Company |
|---|---|---|---|
| SLURP-1 | 0,5 ng/ml; 5 ng/ml | 30 min | Abnova (Heidelberg, GER) |
| Substance P | 10 μM | 30 min | Sigma-Aldrich (München, GER) |
| Nerve growth factor (NGF) | 50 ng/ml | 30 min | Promega (Madison, WI, USA) |
| Albumin, dinitrophenyl, (DNP-HSA) | 30 ng/ml | 30 min | Sigma-Aldrich (München, GER) |
| Ionomycin | 1 μM | 30 min | Sigma-Aldrich (München, GER) |
| Phorbol 12-myristate 13-acetate (PMA) | 10 nM | 30 min co-incubation with Ionomycin | Sigma-Aldrich (München, GER) |