Table 4.

Stressors and stimulators used in cMC cell culture.

Stimulants	Concentrations	Incubation times	Company
SLURP-1	5 ng/ml	2, 8, 24 h 8 h pre-incubation Prior to 24 h IL1β	Abnova (Heidelberg, GER)
AR-R 17779 hydrochloride (Chrna7 agonist)	10 μM	2, 8, 24, 32 h 8 h pre-incubation Prior to 24 h SLURP-1	TOCRIS (Bristol, UK)
IL1β	5 ng/ml	24 h	PeproTech (Hamburg, GER)
PX-478 2HCl (HIF1α inhibitor)	40 μM	8 h pre-incubation Prior to 24 h SLURP-1	Selleckchem (Boston, US)