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. 2021 Mar 31;11:7236. doi: 10.1038/s41598-021-86740-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Serpin concentration and localization in extracts of B. longum NCC 2705, its serpin knock-out counterpart (NCC 9035) and the constitutively producing (NCC 2705 harboring pMDY25) recombinant strain. Means and standard deviations are depicted. Black bars represent cytoplasmic fractions, dark grey bars represent cell-wall associated fractions and light grey represent supernatant fractions (i.e. excreted protein). Serpin amounts are reported in picograms (pg) per mg of total protein.