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. 2021 Mar 31;11:7236. doi: 10.1038/s41598-021-86740-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Serpin concentration measured in extracts of B. longum NCC 2705 grown for 24 (dark grey) and 48 h (light grey) on different monosaccharides (A). Serpin concentration measured in B. longum NCC 2705 grown for 16 h on different concentrations of glucose and galactose (B) and glucose and fructose (C). Bars represent serpin quantity normalized by total protein content, means and standard deviations are depicted. Initial carbohydrate concentrations are depicted. Presence of residual glucose in the culture supernatant after 16 h of incubation is indicated (+ /−) (B,C). Triangles represent growth at the end of the incubation, measured by optical density at 600 nm. P-values represent statistical difference to the glucose control group. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05.