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. 2021 Mar 31;11:7239. doi: 10.1038/s41598-021-86593-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence microscopy of LPC mice validation of shotgun proteomics analysis. (A) Representative images of LPC mice from the sedentary control, exercising animals, and secondary control for connexin-32 (CX32), and myelin (MBP). Scale bar denotes 100 μm. (B) Quantification of lesion area. (C) Quantification of percent of lesion that is MBP-positive. *p < 0.05. (D) Quantification of percent of lesion that is CX32-positive. **p < 0.01. A Mann–Whitney test was used for statistics. Each point is of a single animal, and the bar represents mean ± SD (n of 8 per group).