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. 2021 Apr 1;8:69. doi: 10.1038/s41438-021-00505-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Mature sweet orange branch with full buds, which can be used as scion for grafting. B Annual seedling of Poncirus trifoliata, which can be used as rootstock for grafting. C Cutting the bud. D Cutting the rootstock. E Putting the bud on rootstock. F Binding the graft union with plastic film. G Cutting out the rootstock shoot above the graft union after graft union healing. H The live bud grafted on the rootstock. I New shoot coming out from the grafted bud. S scion, R rootstock