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. 2021 Mar 18;12:614909. doi: 10.3389/fpls.2021.614909

FIGURE 10.

FIGURE 10

The expression levels of six stresses-responsive genes including GmWRKY40, GmMYB84, GmGST15, GmDREB-like, GmbZIP78, and GmNAC29. (A) The expression levels of six stresses-responsive genes were measured using qRT-PCR in transgenic GmCrRLK1L20 soybean hairy root plants under normal conditions. (B) The expression levels of six stresses-responsive genes were measured using qRT-PCR in transgenic GmCrRLK1L20 soybean hairy root plants under drought stresses conditions (200 mM mannitol). (C) The expression levels of six stresses-responsive genes were measured using qRT-PCR in transgenic GmCrRLK1L20 soybean hairy root plants under salt stress conditions (200 mM NaCl). RNAi, EV, and OE represent GmCrRLK1L20-RNAi, EV-Control (the empty plasmid of pCAMBIA3301), and GmCrRLK1L20-OE, respectively. Vertical bars indicate ± SE of three biological replicates. ANOVA test indicated that there were significant differences (*p < 0.05, **p < 0.01).