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. 2021 Mar 18;12:652980. doi: 10.3389/fmicb.2021.652980

FIGURE 4.

FIGURE 4

Ribosome rescue by ArfB. (A) Non-stop complex (large subunit, 50S, gray; small subunit, 30S, yellow) with peptidyl-tRNA (green) in the P-site and mRNA extending two nucleotides into the A-site (short mRNA, cyan) [PDB ID 7JSZ (Carbone et al., 2020)]. (B-D) ArfB (purple) bound to the non-stop complex with the NTD in the collapsed (B) [PDB ID 7JSZ (Carbone et al., 2020)], pre-accommodated (C) [PDB ID 7JSW (Carbone et al., 2020)], as well as fully accommodated (D) [PDB ID 6YSS (Chan et al., 2020)] conformation. The conformation of the NTD is enlarged in the inlays. The C-terminal tail extends into the mRNA channel in each conformation. (E) Ribosome with mRNA extending nine nucleotides into the A-site and the mRNA channel (long mRNA, cyan) and peptidyl-tRNA in the P-site [PDB ID 6YSR (Chan et al., 2020)]. (F) ArfB dimer (ArfB, purple and ArfB-2, pale violet red) on the ribosome with the long mRNA sandwiched between the ArfB dimer and the P-site tRNA [PDB ID 7JT1 (Carbone et al., 2020)]. (G) ArfB or the ArfB dimer dissociated and ribosome recycling had occurred.