Table 2.

Media formulation for 100 ml total volume.

Medium	Formulation (100 ml)
Maintenance of hiPSCs	Essential 8TM Basal medium (Thermo Fischer Scientific)– 98 ml. Essential 8TM Supplement 50X (Thermo Fischer Scientific)–2 ml.
Proneural medium	Essential 6TM medium (Thermo Fischer Scientific)–95ml. Penicillin/Streptomycin (Thermo Fischer Scientific)–100 μl. N2 supplement (Thermo Fischer Scientific)–5 ml.
Maturation medium	DMEM-F12 TM (Thermo Fischer Scientific)–97 ml. B27 Supplement (Thermo Fischer Scientific)–2 ml. MEM Non-Essential Amino Acids Solution 100X (Thermo Fischer Scientific)–1 ml. Penicillin/Streptomycin (Thermo Fischer Scientific)–100 μl.
Ringer Solution	NaCl 155 mM, KCl 5 mM, CaCl2 2 mM, NaCl2 1 mM, NaH2PO4 2 mM, HEPES 10 mM, glucose 10 mM.