. 2021 Mar 18;12:652704. doi: 10.3389/fgene.2021.652704

TABLE 2.

Enzyme kinetic parameters for the N-acetylation of eight drugs using recombinant proteins encoded by NAT2*4, *5, *6, and *7 alleles.

	Km (μM)	Vmax (relative peak area/min/mg protein)	CLint (Vmax/Km)
AGT
*4	76.24 ± 2.49	45.18 ± 1.52	0.593 ± 0.010
*5	104.07 ± 8.65	10.87 ± 0.65^a	0.105 ± 0.004^a
*6	187.75 ± 26.36^a,b	6.70 ± 0.51^a,b	0.037 ± 0.003^a,b
*7	188.83 ± 5.56^a,b	5.44 ± 0.18^a,b	0.029 ± 0.001^a,b
DDP
*4	98.17 ± 8.60	24.73 ± 1.67	0.255 ± 0.018
*5	85.31 ± 10.69	4.86 ± 0.06^a	0.059 ± 0.006^a
*6	15.30 ± 0.44^a,b	0.54 ± 0.03^a,b	0.035 ± 0.001^a
*7	5.21 ± 0.26^a,b	0.96 ± 0.01^a,b	0.185 ± 0.008^a,b,c
HLZ
*4	5.79 ± 0.26	1023.20 ± 33.02	177.09 ± 4.94
*5	4.68 ± 0.19^a	224.78 ± 3.85^a	48.15 ± 1.42^a
*6	2.97 ± 0.17^a,b	45.78 ± 1.65^a,b	15.46 ± 0.41^a,b
*7	4.76 ± 0.26^a,c	28.29 ± 1.38^a,b	5.95 ± 0.10^a,b
INH¹
*4	128.10 ± 4.54	1496.50 ± 57.79	11.706 ± 0.422
*5	139.80 ± 16.33	196.35 ± 8.57^a	1.449 ± 0.126^a
*6	135.20 ± 9.81	61.94 ± 2.22^a,b	0.462 ± 0.019^a,b
*7	230.50 ± 22.29^a,b,c	69.22 ± 2.00^a,b	0.306 ± 0.021^a,b
PZ
*4	192.20 ± 11.70	67.02 ± 5.27	0.348 ± 0.012
*5	134.50 ± 11.59	13.27 ± 0.97^a	0.102 ± 0.014^a
*6	174.35 ± 23.60	7.30 ± 0.38^a	0.044 ± 0.006^a,b
*7	376.68 ± 28.77^a,b,c	6.81 ± 0.07^a	0.018 ± 0.001^a,b
PA
*4	193.13 ± 15.16	78.33 ± 2.19	0.410 ± 0.019
*5	164.65 ± 6.65	19.63 ± 0.28^a	0.120 ± 0.006^a
*6	220.30 ± 9.34^b	17.04 ± 0.25^a	0.078 ± 0.002^a
*7	167.73 ± 4.22^c	10.13 ± 0.24^a,b,c	0.061 ± 0.002^a,b
SMZ
*4	86.24 ± 6.58	4.83 ± 0.17	0.056 ± 0.002
*5	136.00 ± 5.87^a	0.98 ± 0.06^a	0.007 ± 0.000^a
*6	85.95 ± 2.92^b	0.34 ± 0.00^a,b	0.004 ± 0.001^a
*7	12.37 ± 1.15^a,b,c	0.21 ± 0.01^a,b	0.017 ± 0.002^a,b,c
SP
*4	174.90 ± 20.79	63.53 ± 4.73	0.369 ± 0.019
*5	162.70 ± 12.93	13.44 ± 0.17^a	0.084 ± 0.006^a
*6	142.88 ± 14.10	6.18 ± 0.13^a	0.044 ± 0.003^a
*7	27.50 ± 1.10^a,b,c	4.60 ± 0.07^a	0.168 ± 0.010^a,b,c

Each value represents the mean ± SEM of four independent experiments. Tukey’s multiple comparison test; ^aP < 0.05 compared with NAT2^∗4, ^bP < 0.05 compared with NAT2^∗5, ^cP < 0.05 compared with NAT2^∗6. The data were reported previously (Suvichapanich et al., 2018).