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. 2021 Mar 31;11:7290. doi: 10.1038/s41598-021-86886-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Lipid metabolisms change in dormant cancer cells. (a) Cholesterol metabolism involved in 3D cultured S500 and S125 spheroids. S500 spheroids displayed higher cholesterol metabolism than S125 spheroids. Genes were selected from the “Cholesterol metabolism (includes both Bloch and Kandutsch–Russell pathways) (Homo sapiens)” pathway according to Wikipathways. Red indicates increased expression, and green denotes decreased expression. (b) The mRNA expression of acyl-CoA synthetase medium chain family member 3. (c) The mRNA expression of acyl-CoA synthetase short-chain family member 2. All mRNA expression analyses were conducted using Transcriptome Analysis Console ver. 4.0.1.36. Genes with changes in expression exceeding 1.5-fold and significant at p < 0.05 genes were extracted. n = 2.