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. 2021 Mar 31;11:7290. doi: 10.1038/s41598-021-86886-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Triacsin C suppressed protoporphyrin IX (PpIX) accumulation after 5-aminolevulinic acid (ALA) treatment. Cells were treated with triacsin C alone for 48 h and then co-cultured with triacsin C and ALA for 24 h. (a) Confocal laser-scanning microscopy images of DRAQ5 (nuclei) and PpIX. Scale bar, 50 μm. (b) PpIX accumulation in the presence of absence of triacsin C as measured using a microplate reader. n = 3. *p < 0.001. All bars represent the SE.