Figure 2.

Number of PGCs (A, C, E, G) and reprogrammed EGCs (B, D, F, H) under non-reprogramming conditions (except for G&H) and different pharmacological supplementation. (A, C, E, G) No differences were found in PGC numbers in the presence of staurosporine, Ro-31-8220, PIP, and FR236924, demonstrating that these compounds are neither toxic nor induce augmented proliferation in cultures at the concentrations employed. (B) Staurosporine alone induces PGC reprogramming, by a statistically significant increase in reprogrammed EGC colonies. (D) Ro-31-8220 alone induces PGC reprogramming, by a statistically significant increase in reprogrammed EGC colonies. (F) PIP alone induces PGC reprogramming, by a statistically significant increase in reprogrammed EGC colonies. (H) FR236924 does not have an effect on PGC reprogramming under hypoxic conditions. An asterisk represents a statistically significant p value < .05.