Table 1.

Primers used for amplification of DLGAP1, EMILIN2 & MYOM1 gene and their annealing temperature and product size

Gene	Exon	Primer sequence	Ta (°C)	Product size (bp)
MYOM1	4	F; 5’ CATGAAGTTGTTTACACTTCAACTTAC 3’	63	260
		R; 5’ CTCAGTGTGATCACACAGCAT TGG 3’
	19	F; 5’ TGCTTCTACACCTGCTTCTA CAG 3’	56	259
		R; 5’ TTATATTCAGATAGCACACATTGA 3’
	29	F; 5’ CCATTTCCTTTCAACCAGAAAGGG 3’	52	218
		R; 5’ CATACATCTGCATG CCCTCCTGG 3’
EMILIN2	4	F; 5’ TTGGTCAACAGATCAAGACATTGGACC 3’	66.7	300
		R; 5’ GAACGCTCCCCAGACGGTCTTCCAGAG 3’
DLGAP1	2	F; 5’ GTCCACGGCATCCAAGCAGACCAC 3’	67.8	223
		R; 5’ TGTTTTCCTCAGGGACAGGCG 3’F
	4	F; 5’ CTGGAGTCGCAGGCCGTGGAAGCG 3’	67.8	300
		R; 5’ ACATGGGTGGTATCTTGTTCCTGG 3’