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. 2021 Mar 18;9:602450. doi: 10.3389/fcell.2021.602450

Figure 4.

Figure 4

WISH in Ciona transgenic embryos of the DEGs inversely expressed in the OC::Act and OC::Rep conditions. WISH on transgenic embryos at tailbud 22/23 stage electroporated with the control pGsx>GFP2x (A,D,G,J,M,P,S,V), the pGsx>OC::Act (B,E,H,K,N,Q,T,W), or with the Gsx>OC::Rep constructs (C,F,I,L,O,R,U,X). According to RNA-Seq data, all genes are upregulated in OC::Act and downregulated in OC::Rep embryos. Black arrows indicate the territory of the visceral ganglion, where no alteration of gene expression can be observed because it is outside the domain of expression of pGsx (J–L,S–X). Percentages indicate the number of embryos with the corresponding expression profile in each condition as described in Supplementary Table 5. n, number of embryos analyzed in each condition. All embryos are in lateral view and the anterior is on the left.