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. 2021 Mar 18;12:645331. doi: 10.3389/fmicb.2021.645331

FIGURE 2.

FIGURE 2

Avirulence phenotypes of Avr1b-1 knock-out transformants measured by detached leaf inoculations. (A) Soybean leaf lesions 3 days after zoospore inoculation of Williams (rps) or L77-1863 (Rps1b) unifoliate leaves. (B) Sizes of lesions after 3 days. Lesions on eight unifoliate leaves were measured in each of three independent experiments. The relative lesion size was measured from photographs with ImageJ software. Lesion sizes were compared between Williams (rps) and L77-1863 (Rps1b) using the Wilcoxon rank sum test. ***P < 0.001, n.s. P > 0.1. (C) Avirulence phenotypes of Avr1b-1 knock-out transformants measured by real-time PCR-based quantification of P. sojae genomic DNA in infected tissue. Proliferation of P. sojae was plotted as the ratio between the abundance of PsActin relative to GmActin DNA in infected L77-1863 leaves compared with that in infected Williams leaves, at 72 hpi. Error bars represent standard deviation of three technical replicates. This experiment was replicated three times. Error bars represent the mean ± standard deviation. P-values were determined by the Wilcoxon rank sum test; *** indicates P < 0.001.