FIGURE 3.

Virulence phenotypes of Avr1b-1 knock-out transformants on Williams soybean leaves. (A) Avr1b-1-silenced isolate P6497; Avr1b-1-expressing isolate P7063; three homozygous Avr1b-1 deletion transformants Δ114-1-7, Δ114-1-8, and Δ114-2-2; plus non-HDR transformant Δ114-1-11f were inoculated onto unifoliate leaves of Williams (rps1b). Pictures were taken 3 days after inoculation. (B) Average lesion sizes determined from at least 10 inoculated unifoliate leaves in each of three independent experiments. The relative lesion sizes were measured by ImageJ software. Error bars represent the mean ± standard deviation. P-values were determined by the Wilcoxon rank sum test. (C) Virulence phenotypes of Avr1b-1 knock-out transformants measured by real-time PCR-based quantification of P. sojae genomic DNA in infected tissue. Error bars represent standard deviation of three technical replicates. This experiment was replicated three times. Error bars represent the mean ± standard deviation.