Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 18;12:645331. doi: 10.3389/fmicb.2021.645331

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Gu, Shao, Gao, Miao, Wang, Liu and Tyler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

G418 sensitivity of Avr1b-1 knock-out transformants. (A) Wild-type P7063 and three selected Avr1b-1 deletion transformants cultured on clarified V8 medium with or without 50 μg/ml G418. Transformants Δ114-1-7 and Δ114-2-2 were isolated from G418 regeneration medium and then subcultured in V8 medium. Transformant Δ114-1-8 was maintained on V8 medium containing 50 μg/ml G418. (B) PCR detection of npt II sequences in genomic DNA and RNA of Avr1b-1 knock-out transformants and P7063 with primer pair qNptF/R. P. sojae Actin was used as the reference gene.