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. 2021 Mar 18;12:645331. doi: 10.3389/fmicb.2021.645331

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Copyright © 2021 Gu, Shao, Gao, Miao, Wang, Liu and Tyler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transient expression of Avr1b-1^sgR leads to cell death in Rps1b soybean leaves. (A) Soybean leaves from Williams (rps1b) and L77-1863 (Rps1b) were subjected to double-barreled particle bombardment with pairs of samples containing GUS reporter DNA plus either Avr1b^sgR DNA or GFP control DNA. After staining, the numbers of blue spots on Williams (rps1b) and L77-1863 (Rps1b) leaves were counted. A functional Avr1b-1 gene is expected to significantly reduce the number of blue spots on L77-1863 (Rps1b) leaves, but not Williams (rps1b) leaves, due to triggering Rps1b-mediated cell death. The white dotted line separates the two bombardment sites. Numbers of GUS spots counted are indicated. (B) Ratios between the numbers of GUS spots produced in the presence of Avr1b-1 vs. control GFP DNA on each leaf, averaged from 10 soybean leaves, in a single experiment. Error bar represents the mean ± standard deviation. The significance of the difference between Williams and L77-1863 leaves was determined by the Wilcoxon rank sum test (Gu et al., 2011), ***P < 0.001.