TABLE 1.

Silencing of Avr1b-1 locus in homozygous Avr1b^sgR knock-in transformants.

Recipienta	HDR constructsb	G418R coloniesc	Homozygous KI transformantsd	Avirulent on Rps1b	Virulent on Rps1b	Silencing of Avr1b-1 locuse
P7063	Avr1bsgR-AVI	32	13	2	11	11/13
	Avr1bsgR-mCherry	34	15	1	13	13/15
Δ114-1-7	Avr1bsgR-AVI	33	11	2	9	9/11
	Avr1bsgR-mCherry	30	9	0	9	9/9

^aP. sojae wild-type P7063, Avr1b-1 knock-out transformant Δ114-1-7.

^bHomology-directed recombination constructs used to produce Avr1b^sgR knock-in transformants, consisting of AVI- or mCherry-tagged Avr1b^sgR fused to 1 kb flanking sequence at each end.

^cRegenerated colonies exhibiting vigorous growth in V8 broth containing 50 μg/ml G418.

^dHomozygous Avr1b-1^sgR or mCherry knock-in transformants verified by PCR and sequencing.

^eRatio of silenced mutants compared with total homozygous transformants. Abundance of transcripts from the Avr1b-1 locus was evaluated by RT-PCR as in Figure 7D and Supplementary Figure S7.