Skip to main content
. 2021 Mar 18;12:642173. doi: 10.3389/fimmu.2021.642173

Figure 2.

Figure 2

SOCS3 expression by non-hematopoietic cells is central for thymus maintenance and T cell differentiation. (A) Radiation bone marrow (BM) chimeras were generated using WT and Δsocs3 mice as recipients or donors. Sixty days after transplantation, mice were treated with Tm and sacrificed 7 days after the last dose. (B) The mean thymic cell numbers ± SEM is depicted (n ≥ 5 per group). Differences between groups are significant at *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.01 Welch one way ANOVA. (C) Representative dot plots and (D–G) mean frequencies of DN, DP, CD4 SP, and CD8 SP thymocytes ± SEM in BM chimeric Δsocs3 and WT mice are depicted (n ≥ 5 per group). Differences between groups are significant at *p ≤ 0.05, **p ≤ 0.01 Welch one-way ANOVA. (H,I) The mean frequency of (H) γδ and (I) β TCR+ cells within DN ± SEM BM chimeric mice is depicted. Differences between groups are significant at *p ≤ 0.05 Welch one-way ANOVA. (J) The mean % IL-7R+ cells within DN population ± SEM in BM chimeras of Δsocs3 and WT is shown.