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. 2021 Mar 18;12:642173. doi: 10.3389/fimmu.2021.642173

Figure 5.

Figure 5

Non-hematopoietic SOCS3 expression regulates the frequency recent thymic emigrants and of naïve peripheral T cells. (A) WT or Δsocs3 mice treated with Tm and 7 days after the last dose of Tm were irradiated and transplanted with CD45.1+ WT bone marrow cells. Mice were sacrificed 60 days after BM transplantation. (B) The mean number of thymus, spleen and lymph node cells ± SEM of transplanted mice are depicted (n = 5 per group). The mean number ± SEM of CD45.1+ and CD3 gated CD4, CD8, and γδ T cells in (C) spleens and (D) lymph nodes of radiation chimeric mice treated as described above is shown (n = 5 per group). The mean MFI of CD44 in CD45.1 gated CD4 or CD8 T cells in spleens (E) and lymph nodes (F) is shown. (G) A representative histogram of CD44 in CD8 T cells in lymph node or spleen cells from Δsocs3 and WT mice. The mean MFI and representative histograms of Qa-2 (H–K) and CD24 (L–O) expression in naïve CD4 (H,I,L,M) and CD8 (J,K,N,O) splenic T cells of WT and Δsocs3 mice 7 days after Tm treatment are depicted. Differences between groups are significant at *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, unpaired Student's t-test.