Table 1.
List of some of the viruses detected by ddPCR
| Method | Virus | Finding | References |
|---|---|---|---|
| One‐step RT‐ddPCR | SVA | LOD is 10 times lower than that of an RT‐qPCR. | 161 |
| ddPCR | HTLV‐1 | – | 151 |
| Two‐step RT‐ddPCR | HPeV3 | Two‐step RT‐ddPCR was less variable and more specific than one‐step RT‐ddPCR. | 162 |
| RT‐ddPCR | JEV | ddPCR had a high degree of linearity, high specificity, and JEV RT‐ddPCR was more sensitive than real‐time RT‐PCR. | 163 |
| ddPCR | RSV | – | 139 |
| Micro RT‐ddPCR | Zika Virus | ddPCR has outstanding accuracy and sensitivity for samples of low concentrations. | |
| RT‐ddPCR | dengue virus serotype 2 | RT‐ddPCR assay developed had similar specificity to the routine RT‐qPCR assay. | 164 |
| RT‐ddPCR | Zika virus | – | 73 , 166 |
| ddPCR | Anelloviruses | – | 166 |
| ddPCR | HCMV, herpes simplex virus, EBV, and varicella‐zoster virus | The results of ddPCR were consistent with that of next‐generation sequencing. Compared with qPCR, results of ddPCR showed better consistency with the validity of clinical treatment. | 86 |
Abbreviations: EBV, Epstein‐Barr virus; HCMV, human cytomegalovirus; HPeV3, human par echovirus type 3; HTLV‐1, human T‐cell leukemia virus, type 1; JEV, Japanese encephalitis virus; LOD, limit of detection; RSV, respiratory syncytial virus; SVA, seneca virus A.