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. 2020 Dec 23;12:1758835920982853. doi: 10.1177/1758835920982853

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© The Author(s), 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Knockdown of ATR by siRNA inhibits ovarian cancer cell viability and proliferation. (A) Cell viability of ovarian cancer cells as measured by MTT assay after ATR-specific siRNA transfection (**p < 0.01, ***p < 0.001). (B) Cell proliferation was decreased, and representative images of ovarian cancer cell morphologic changes after ATR siRNA transfection are presented. Original magnification value, ×100. Scale bar 1000 µm. (C) ATR expression levels and related signaling pathway proteins involved in DNA damage, cell cycle arrest, and apoptosis after transfection of ATR siRNA and nonspecific siRNA in SKOV3 and OVCAR8 cell lines via western blot.

ATR, ataxia-telangiectasia and Rad3 related; Chk1, checkpoint kinase 1; MTT, methyl thiazolyl tetrazolium; p-ATR, phospho-ATR ser428; p-Chk1, phosphorylated Chk1.