Figure 7.

ATR inhibitor VE-822 reduced ovarian cancer cell viability and proliferation via phosphorylation of ATR and Chk1. (A) Cell viability was measured by MTT after treatment with VE-822 at increasing concentrations. (B) Cell proliferation was inhibited, and representative images of ovarian cancer cell morphologic changes after VE-822 treatment are shown. Original magnification value, ×100. Scale bar 1000 µm. (C) ATR expression levels and related signaling pathway proteins involved in DNA damage, cell cycle arrest, and apoptosis after VE-822 treatment in SKOV3 and OVCAR8 cell lines by Western blot.

ATR, ataxia-telangiectasia and Rad3 related; Chk1, checkpoint kinase 1; MTT, methyl thiazolyl tetrazolium; p-ATR, phospho-ATR ser428; p-Chk1, phosphorylated Chk1.