Table 2.

Limits of quantitation and dynamic range of analytes measured by the bead-based fluorescence (LMX) and planar electrochemiluminescence (MSD) multiplex assays. Dynamic range spans the lower limit of quantitation (LLoQ) and upper limit of quantitation.

19 shared analytes	LMX		MSD		Range size ratio LMX/MSD
	Dynamic range (pg/mL)	Dynamic range size (fold of LLoQ)	Dynamic range (pg/mL)	Dynamic range size (fold of LLoQ)
GM-CSF	0.90-18 629	20 699	0.05-4393	87 860	0.23
IFN-γ	0.19-9639	50 731	0.29-4745	16 362	3.10
IL-1β	0.21-7315	34 833	0.03-1791	59 700	0.58
IL-2	0.20-7633	38 165	0.07-5401	77 157	0.49
IL-4	1.49-28 992	19 458	0.01-761	76 100	0.26
IL-5	0.20-7581	37 905	0.08-3465	43 312	0.87
IL-6	0.66-2912	4412	0.04-2524	63 100	0.07
IL-7	3.62-5717	1579	0.06-3397	56 617	0.03
IL-8	0.10-4795	47 950	0.02-2277	113 850	0.42
IL-10	1.21-23 291	19 249	0.08-1806	22 575	0.85
IL-12 p70	0.27-7909	29 293	0.03-2217	73 900	0.40
IL-13	0.20-3913	19 565	0.11-1574	14 309	1.37
IL-17A	1.78-11 391	6399	0.23-20 872	90 748	0.07
IL-21	0.11-3818	34 709	0.22-5786	26 300	1.32
IL-23	12.15-124 034	10 208	0.22-20 833	94 695	0.11
MIP-1α	5.58-4604	825	1.17-4345	3714	0.22
MIP-1β	0.57-14 808	25 979	0.30-3528	11 760	2.21
MIP-3α	0.60-9400	15 667	0.08-1806	22 575	0.69
TNF-α	0.08-6529	81 612	0.07-1362	19 457	4.19
Unshared analytes	Dynamic range (pg/mL)		Dynamic range (pg/mL)
Fractalkine	19.38-285 596	14 737	n/a	n/a	n/a
ITAC	1.58-22 425	14 193	n/a	n/a	n/a
IL-8 HA	n/a	n/a	109-239 574	2198	‒

“n/a” indicates “not applicable” because that particular analyte was not included in the platform.