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. 2021 Mar 18;12:632132. doi: 10.3389/fimmu.2021.632132

Figure 2.

Figure 2

Protective effects of (S S)-DS-ONJ against LPS-mediated iNOS activation and elevation of mRNA levels of pro-inflammatory cytokines in Bv.2 microglial cells. Bv.2 microglial cells were treated for 24 h with LPS (200 ng/mL) or LPS plus (S S)-DS-ONJ (1–10 μM). (A) Colorimetric quantification of nitrites was performed. (B) mRNA of Nos2 was determined by qRT-PCR. (C) Protein extracts were analyzed by Western blot with the corresponding antibodies against iNOS and α-tubulin as loading control. Representative autoradiograms are shown. Blots were quantified by scanning densitometry and the results are presented as mean ± SEM. (D) Tnfa, Il1b, and Il6 mRNA values were determined by qRT-PCR. The results are presented as means ± SEM (n = 6 independent experiments). Fold changes are calculated relative to the basal value. *p ≤ 0.05 vs LPS treatment, †p ≤ 0.05 vs basal value (two-way ANOVA followed by Bonferroni t-test.).