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. 2021 Mar 18;12:632132. doi: 10.3389/fimmu.2021.632132

Figure 4.

Figure 4

Protective effects of (S S)-DS-ONJ against LPS-mediated inflammasome activation in Bv.2 microglial cells. (A) Nlrp3 mRNA was measured by qRT-PCR. (B) Protein extracts were analyzed by Western blot, with antibodies against NLRP3; α-tubulin was used as loading control. (C) Protein extracts were analyzed by Western blot, with antibodies against caspase-1; α-tubulin was used as loading control. Representative autoradiograms are shown (n = 6 independent experiments). Blots were quantified using scanning densitometry, and the results are presented as means ± SEM. The results are presented as means ± SEM (n = 6 independent experiments). Fold changes are calculated relative to the basal values. *p ≤ 0.05 vs LPS treatment, †p ≤ 0.05 vs basal values (two-way ANOVA followed by Bonferroni t-test).