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. 2021 Mar 18;11:643163. doi: 10.3389/fonc.2021.643163

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Copyright © 2021 Zhang, Zhu, Xiao, Cheng, He, Liu, Xiang, Gong, Wang, Deng and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TMPO-AS1 promotes the proliferation, migration, invasion, and survival of BC cells in vitro. (A) The qRT-PCR show the mRNA levels of TMPO-AS1 in five BC cell lines (BIU87, 5637, T24, EJ, and RT4). (B) The efficiencies of TMPO-AS1 knockdown or overexpression in RT4 and T24 cells were examined by qRT-PCR. Mock, the negative control; Si-TMPO-AS1, siRNA targeting TMPO-AS1; OE-TMPO-AS1, ectopic expression of TMPO-AS1. (C,D) The effect of TMPO-AS1 knockdown and overexpression on the cell proliferation was measured using methyl thiazolyl tetrazolium (MTT) and colony formation assays. (E,F) The effect of TMPO-AS1 knockdown or overexpression on the migration and invasion of RT4 and T24 cells was evaluated via wound healing and transwell assays. (G) Cell apoptosis was analyzed by flow cytometry in TMPO-AS1 knockout or overexpressing RT4 and T24 cells, stained with Annexin V-PE/7-AAD. Error bars represent the mean ± SD from three independent experiments. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.