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. 2021 Mar 18;11:643163. doi: 10.3389/fonc.2021.643163

Figure 7.

Figure 7

TMPO-AS1 promotes BC malignant phenotypes via E2F1 in vitro. (A) The TMPO-AS1 expression was evaluated using qRT-PCR in RT4 and T24 cells transfected with an empty vector, sh-TMPO-AS1, or co-transfected with sh-TMPO-AS1 and E2F1-ectopic expression vector. (B,C) MTT and colony formation assays were performed to assess the proliferative ability of transfected cells. (D,E) The cell migration and invasion were estimated using wound healing and transwell assays. (F) The apoptosis in transfected BC cells stained with Annexin V-PE/7-AAD was evaluated via flow cytometry analysis. Error bars represent the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.