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. 2021 Feb 22;40(7):e106065. doi: 10.15252/embj.2020106065

Figure EV3. 5‐FU treatment leads to the appearance of micronuclei‐like DNA structures.

Figure EV3

  1. Ctrl MC38 cells stably expressing GFP were treated with 0.3 µM 5‐FU, 300 µg/ml DTIC or PBS for 48 h. The cells were stained with DAPI (blue). Representative pictures are shown, with enlarged areas indicated by white boxes. Arrows point to examples of micronuclei‐like DNA structures.
  2. Quantification of micronuclei‐like DNA structures per 100 nuclei. N = 10 fields.
  3. Ctrl MC38 cells were treated in vitro with the indicated concentrations of DTIC for 2 days. Cell viability was determined using the CellTiter‐Glo assay and normalized based on the level of PBS treatment. N = 3.

Data information: For all panels, error bars stand for SD, and center values represent mean. Two‐tailed unpaired Student’s t‐test was used. ****P < 0.0001; ns: not significant.