Figure 4. Cancer‐cell‐intrinsic cGAS is required for efficient 5‐FU‐induced reduction of tumor burden.

• A
  Western blot for control sgRNA (Ctrl) and cGAS‐KO MC38 cells, assayed with cGAS and GAPDH antibodies.
• B–D
  Mice were injected with Ctrl or cGAS‐KO MC38 cells and treated with PBS or 5‐FU. (B) Pictures of tumors and spleens from a representative experiment. Image panels were cropped from the same picture. (C) Tumor volumes were quantified at the indicated days after cancer cell injection. N = 4 to N = 5, as shown in (B). (D) Tumor and spleen weights at the endpoint for (C), with each dot representing a mouse.

Data information: For all panels, error bars stand for SD, and center values represent mean. Two‐tailed unpaired Student’s t‐test was used. *P < 0.05; ****P < 0.0001; ns: not significant. Data are representative of two or more independent experiments.