Skip to main content
. 2021 Jan 25;40(7):e106745. doi: 10.15252/embj.2020106745

Figure 2. CRY‐less oscillations are temperature‐compensated and entrained.

Figure 2

  1. Detrended traces of bioluminescence recordings of WT and CKO fibroblast at different constant temperature conditions within the physiological range (n = 4, solid lines: mean, dashed lines: ± SEM). Temperature was changed from 37°C to 32°C halfway through the experiment, as depicted by red/blue shading. Arrows represent medium changes. Note the lack of rhythmicity in the first 3 days in CKO and the appearance of rhythmicity after the first medium change.
  2. Quantification of period from recordings presented in (A). Both WT and CKO oscillations are temperature‐compensated with respective Q10s of 1.05 and 0.95 (n = 3, mean ± SEM). P‐values were determined by two‐tailed t‐test.
  3. Bioluminescence of WT and CKO PER2::LUC cells during temperature entrainment (12 h 32°C (blue)–12 h 37°C (red)) (n = 3, solid lines: mean, dashed lines: ± SEM).