Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 17;12:634185. doi: 10.3389/fmicb.2021.634185

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Li, Bai, Liu, Zhang, Liu, Zhang, Xu, Yang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Serine protease affects the expression of TJ proteins. The Caco-2 cells were treated with TNF-α (20 ng/ml), Ts-ML-ES (25 μg/ml), heat-inactivated Ts-ML-ES (25 μg/ml), or PMSF pretreated Ts-ML-ES (25 μg/ml). (A) Expression of Claudin-1, Occludin, and ZO-1 mRNA. Data represent the mean ± SD from the mean of three independent experiments. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by one-way ANOVA with Tukey’s posttest. (B) Protein expression of Claudin-1, Occludin, and ZO-1. β-actin was used as the internal control. Representative blots of three independent experiments are shown. (C) IFA of Claudin-1, Occludin, and ZO-1. The merged images represent the three TJ proteins in green. DNA was stained with DAPI (blue) to reveal the positions of the nuclei.