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Primer sequences and gene fragment size of amplified genes.
| Gene name | Primer sequence(5'-3') | Product size |
|---|---|---|
| GAPDH | F: CTCCTCCTGTTCGACAGTCA | 106 bp |
| R: CGACCAAATCCGTTGACTCC | ||
| Claudin-1 | F: TGGTCAGGCTCTCTTCACTG | 119 bp |
| R: TTGGATAGGGCCTTGGTGTT | ||
| Occludin | F: GGGCATTGCTCATCCTGAAG | 85 bp |
| R: GCCTGTAAGGAGGTGGACTT | ||
| ZO-1 | F: TTCACGCAGTTACGAGCAAG | 141 bp |
| R: TTGGTGTTTGAAGGCAGAGC |
