FIGURE 5.

Thermostability (A) and optimal temperature (B) of AaSOD. For thermostability measurement, the purified AaSOD was incubated at different temperatures (RT, 60, 70, 80, 90, and 100°C) for 1 h, and subsequently measured the residual activities. The relative activity was calculated as the percentage of the maximum activity at 35°C. To achieve the optimal catalytic temperature, the activity of purified AaSOD was tested at different temperatures (25–80°C) and the highest activity was set as 100% for relative activity calculation.