TABLE 1.

Superoxide dismutase (SOD) activity incorporating various ions.

Sample	Specific activity (U⋅mg–1)
Natural medium	90.94 ± 0.47
AaSOD from Fe2+ enriched medium	74.65 ± 2.92
AaSOD from Mn2+ enriched medium	1198.55 ± 20.36
AaSOD from Fe2+ and Mn2+ enriched medium	534.56 ± 27.33
Apo-enzyme	5.50 ± 0.40
Fe2+-reconstitution	21.05 ± 1.10
Mn2+- reconstitution	517.63 ± 48.35
Fe2+/Mn2+- reconstitution	270.27 ± 127.83

The apo-enzyme was prepared by dialyzing the purified AaSOD with 50 mM acetate buffer (pH 3.8) containing 8 M urea and 10 mM EDTA.