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. 2021 Mar 18;12:643499. doi: 10.3389/fpls.2021.643499

FIGURE 5.

FIGURE 5

ALSV VIGS can be transmitted to progeny plants through seeds at a low frequency. (A) A representative image of progeny seedlings with the photobleaching phenotype at 7 dpg, derived from plants inoculated with ALSV-CqPDSC in the inbred Iw line. Arrowheads indicate progeny seedlings with the photobleaching phenotype. (B) Semi-quantitative RT-PCR of ALSV RNA1 and RNA2 in the progeny seedlings with (white) or without (green) the photobleaching phenotype shown in (A). CqEF1α was used as an internal control. Arrowheads indicate the positions of recombinant ALSV RNA2 with the trigger sequence (top) and WT RNA2 without the trigger sequence (bottom). (C) An arrowhead indicates a progeny seedling that has not accumulated betalain in the hypocotyl at 14 dpg, derived from J056 inbred line plants inoculated with ALSV-CqDODA1. (D,E) Representative images of a plant with a green hypocotyl (D) or red hypocotyl (E) at 4 wpg, derived from J056 inbred line plants inoculated with ALSV-CqDODA1. (F) Semi-quantitative RT-PCR of ALSV RNA1 and RNA2 in progeny seedlings with a green or red hypocotyl shown in (D,E). CqUBQ10 was used as an internal control. Arrowheads indicate positions of recombinant ALSV RNA2 with the trigger sequence (top) and WT RNA2 without the trigger sequence (bottom). (G) A representative image of a progeny plant with chlorotic spots in leaves at 20 dpg, derived from the Iw inbred line plants inoculated with ALSV-WT. Arrowheads point to chlorotic spots in leaves in progeny seedlings with ALSV-WT. Scale bars represent 1 cm.