Table 1.

Comparison of the original C _t for positive DTS tested with three commercial platforms with their corresponding C _t in pooled samples

	LightMix E‐gene assay			Xpert Xpress SARS‐CoV‐2 assay			Panther Fusion SARS‐CoV‐2 assay
Pool No.	C t of pool	C t of original	ΔC t	C t of pool (E‐gene/N2 gene)	C t of original (E‐gene/N2 gene)	ΔC t (E‐gene/N2 gene)	C t of pool (ORF 1ab)	C t of original(ORF 1ab)	ΔC t
1	18.08	15.55	2.53	17.8/20.5	15.3/18.1	2.5/2.4	25.0	16.5	8.5
2	21.81	19.32	2.49	22.2/25.0	22.5/26.7	−0.3/−1.7	26.5	29.2	−2.7
3	29.55	27.11	2.44	29.2/31.9	26.7/29.0	2.5/2.9	30.9	27.9	3.0
4	35.00	35.00	0.00	37.3/40.3	Negative/37.4	−/2.9	Negative	35.6	–
5	30.83	28.62	2.21	32.9/36.1	28.5/31.4	4.4/4.7	32.7	38.1	−5.4
6	22.04	20.62	1.42	21.5/23.7	20.5/22.7	1.0/1.0	22.9	22.2	0.7
7	34.49	29.43	5.06	34.3/38.0	30.5/33.1	3.8/4.9	35.5	38.1	−2.6
8	25.47	23.05	2.42	23.8/26.8	21.2/24.1	2.6/2.7	24.4	21.8	2.6
9	28.50	27.51	0.99	26.5/29.3	24.4//26.9	2.1/2.4	30.4	30.2	0.2
10	29.65	29.03	0.62	28.6/31.6	26.2/29.1	2.4/2.5	35.9	36.6	−0.7
11	25.59	22.03	3.56	24.7/27.3	21.2/24.1	3.5/3.2	30.2	34.1	−3.9
ΔC t mean (range)	2.16 (0.00–5.06)			2.5/2.5 (−0.3 to 4.4/−1.7 to 4.9)			0.0 (−5.4 to 8.5)

Abbreviations: DTS, deep throat saliva; SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2.

Note: Remark: ΔC _t = C _t of pool − C _t of original

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