Figure 4.

Immunoreactive levels and expression of TMPRSS2 in the lungs, LV, RV and kidneys of compensated, decompensated CHF and sham controls as were determined with western blots analysis and RT‐qPCR. A, Representative western‐blot analysis of tissue lysates with antibody for TMPRSS2. Western‐blot analysis quantification of TMPRSS2 in the lungs, LV, RV and kidneys (B, D, F and H, respectively) of sham, compensated CHF and decompensated CHF (n = 3‐10), where GAPDH was used as loading control. Quantification of RT‐qPCR analysis for TMPRSS2 mRNA normalized to GAPDH are depicted in C, E, G and I. Values are means ± SEM