Validation of transfection efficiency of Nrf-2 overexpressed or low-expressed plasmids in human trophoblast cells (HTR-8/SVneo) and human normal endometrial stromal cells (hESC). A, B. The analysis results of RT-qPCR for Nrf-2 in HTR-8/SVneo and hESC cells after transfected with siRNA-Nrf-2-1 and siRNA-Nrf-2-2 plasmids. C, D. Western blot images and quantitative analyses of Nrf-2 in HTR-8/SVneo and hESC cells. E, F. The analysis results of RT-qPCR for Nrf-2 in HTR-8/SVneo and hESC cells after transfected with siRNA-Keap-1 and siRNA-Keap-2 plasmids. G, H. Western blot images and quantitative analyses of Nrf-2 in HTR-8/SVneo and hESC cells. *P<0.05, **P<0.01 and ***P<0.001 vs. Normal; #P<0.05, ##P<0.01 and ###P<0.001 vs. siRNA-NC.