Figure 5.

Characterization of virus‐based vaccines by nFCM. a) Depiction of the main production steps for virus‐based vaccines. b) Bivariate dot‐plot of FL versus SSC for preparation #A of the PEDV vaccine. c) Bivariate dot‐plot of FL versus SSC for preparation #A of the PRV vaccine. d) Viral concentration and purity of complete virions upon harvest, first purification, and second purification of the PEDV vaccine. e) Comparison between the virus concentration of PEDV and PRV measured by the TCID₅₀ assay and nFCM method; data are represented as the mean ± SD (n=3). The dilution factor of the samples was 100, and the data acquisition time was 1 min for each sample.