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. 2021 Feb 12;9:1248. Originally published 2020 Oct 15. [Version 2] doi: 10.12688/f1000research.26872.2

Figure 1. Single-category annotation of images in multi-dimensional stacks.

Figure 1.

( A) Example of multi-dimensional image stack used to annotate mitotic stages in time-lapse data (source: ImageJ example image “Mitosis” – image credit NIH). ( B) Graphical interface of the single class (buttons) plugin configured for annotation of 4 mitotic stages. ( C) Results table with annotated categories (column category) generated by the plugin after selecting the single category column option in the plugin configuration window (not shown). ( D) Alternative results table output using 1-hot encoding after selecting the option 1 column per category. The resulting 1-hot encoding of categories can be used for the training of classification algorithms.