Table 1.
Comparison of CRISPR‐Cas12 versus Cas13 nucleic acid detection
| Cas12 | Cas13 |
|---|---|
| Cis cleavage | |
| ssDNA and dsDNA 19 | ssRNA 20 |
| At specific bases 19 | At specific bases 20 |
| Distal to target sequence 21 | Distal to target sequence 20 |
| Target or bystander ssDNA 21 | Bystander ssRNA 20 |
| Trans cleavage | |
| Across entire ssDNA (shredding, Lachnospiraceae bacterium, LbCas12a) 21 | At specific bases 20 |
| Thermostable (Alicyclobacillus acidiphilus, AapCas12b) | Works at room temperature 22 |
| Features | |
| nM limit of detection (LoD) | nM LoD 20 |
| aM LoD after PCR 21 , 23 | aM LoD after PCR 20 |
| >90% sensitivity 21 | >95% sensitivity 17 , 20 , 24 , 25 , 26 , 27 |
| ~100% specificity 21 | >99% specificity 17 , 20 , 24 , 25 , 26 , 27 |
| Pioneering diagnostic assays | |
| One‐hour low‐cost multipurpose highly efficient system (HOLMES) 23 | SHERLOCK 20 , 25 |
| Specific high‐sensitivity enzymatic reporter unlocking (SHERLOCK) 25 | |
| DNA endonuclease‐targeted CRISPR trans reporter (DETECTR) 21 |