Figure 5.
(A) Scheme of droplet separation and extraction for liquid chromatography–mass spectrometry (LC–MS) analysis. (From left to right: droplet spotting; the incubation step for allowing permeation; droplet separation; droplet aspiration; placing the droplet in a storage place to evaporate the remaining water/solvents and, following this, a defined volume of water/acetonitrile (2:1) is added for redissolution; LC–MS analysis). (B) Permeation of rhodamine 6G across droplet interface bilayer (DIB) membranes at pH 6.0 measured by MS (N = 4). (Black lines: fits according to eqs 1 and 2 (Papp,fit). (C) Apparent permeability coefficients (N = 27 for Papp,fit fluorescence; N = 4 for MS measurements).