Distribution of eIF4G2 protein in lumbar dorsal root ganglion (DRG) of naïve mice. (a, b) Double-label immunofluorescent staining revealed co-localisation of eIF4G2 (red) with β-tubulin III (green; a), but not with glutamine synthetase (GS; green; b), in the cytoplasm of DRG cells. (b) Cellular nuclei were labelled by 4′,6-diamidino-2-phenylindole (DAPI; blue). (c) Size distribution of eIF4G2-positive neuronal somata in naïve DRG. Large, 41%; medium: 42%; small: 17%. (d–f) Double-labelled immunofluorescent staining (arrows) of eIF4G2 (red) with isolectin B4 (IB4; green), calcitonin gene-related peptide (CGPR; green) or neurofilament 200 (NF200; green) in DRG neurones. n=3 mice. Scale bar, 50 μm. eIF4G2, eukaryotic initiation factor 4 gamma 2.