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. 2020 Dec 7;126(3):706–719. doi: 10.1016/j.bja.2020.10.032

Fig 2.

Fig 2

Peripheral nerve injury-induced increase in expression of eIF4G2 mRNA and eIF4G2 protein in injured dorsal root ganglion (DRG). (a) Levels of eIF4G2 mRNA in the ipsilateral L4 DRG on days 3, 7, and 14 after spinal nerve ligation (SNL) or sham surgery. Four unilateral L4 DRGs from four mice were pooled together. n=3 biological repeats (12 mice) per group per time point. Two-way analysis of variance (anova) with repeated measure followed by post hoc Tukey test. Fgroup (1, 16)=174. ∗∗P<0.01 vs the sham group at the corresponding time point. (b, c) eIF4G2 protein expression in the ipsilateral L4 DRG on days 3, 7, and 14 after SNL or sham surgery. (b) Representative Western immunoblots. (c) Statistical summary of the densitometric analysis. Four unilateral L4 DRGs from four mice were pooled together. n=3 biological repeats (12 mice) per group per time point. Two-way anova with repeated measure followed by post hoc Tukey test. Fgroup (1, 16)=33. ∗∗P<0.01 vs the sham group at the corresponding time point. (d–f) Expression of eIF4G2 protein in the contralateral (Control) L4 DRG (d, e), ipsilateral (Ipsi) L3 DRG (d, e) and ipsilateral L4 spinal cord dorsal horn (SC; f) on days 3, 7, and 14 after SNL. Four unilateral L4 DRGs from four mice were pooled together. n=3 biological repeats (DRGs from 12 mice and spinal cord from three mice) per group per time point. One-way anova with repeated measure followed by post hoc Tukey test. Ftime (3, 8)=0.70 for contralateral L4 DRG, 0.01 for ipsilateral L3 DRG and 0.02 for ipsilateral L4 SC. (g) Expression of eIF4G2 in the ipsilateral L3 and L4 DRGs on day 7 after CCI or sham surgery. Two unilateral L3 and L4 DRGs from two mice were pooled together. n=3 biological repeats (six mice) per group per time point. Two-way anova with repeated measure followed by post hoc Tukey test. Fgroup (1, 8)=25. ∗∗P<0.01 vs the sham group at the corresponding time point. (h–j) Neurones labelled by eIF4G2 in the contralateral (h) and ipsilateral (i) L4 DRG on day 7 after SNL. (h, i) Representative immunostaining images. (j) Statistical summary of the number of eIF4G2-labelled neurones. n=3 mice. ∗∗P<0.01 vs the contralateral side by two-tailed unpaired Student's t-test. Scale bar, 50 μm. CCI, chronic constriction injury; eIF4G2, eukaryotic initiation factor 4 gamma 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.