Skip to main content
. 2020 Dec 7;126(3):706–719. doi: 10.1016/j.bja.2020.10.032

Fig 3.

Fig 3

Effect of dorsal root ganglion (DRG) microinjection of eIF4G2 siRNA on spinal nerve ligation-induced nociceptive hypersensitivities and dorsal horn central sensitisation during the development period. (a) Expression of eIF4G2 protein in the ipsilateral L4 DRG on day 5 after SNL or sham surgery in mice microinjected with eIF4G2 siRNA (Si) or negative control siRNA (NC). n=3 biological repeats (12 mice) per group. Two-way analysis of variance (anova) with repeated measures followed by post hoc Tukey test. Fgroup (1, 8)=42. ∗∗P<0.01 vs the NC plus sham group. ##P<0.01 vs the NS plus SNL group. (b–h) Effect of micoinjection of eIF4G2 siRNA (Si) or negative control siRNA (NC) into the ipsilateral L4 DRG on PWF to 0.07 g (b and f) and 0.4 g (c and g) von Frey filament stimuli and on paw withdrawal latency (PWL) to heat (d and h) and cold (e) stimuli on the ipsilateral (b–e) and contralateral (f–h) sides at the different days after SNL or sham surgery. n=8 mice per group. Two-way anova with repeated measures followed by post hoc Tukey test. Fgroup (3, 112)=50 for 0.07 g, 14 for 0.4 g, 55 for heat, and 49 for cold. ∗∗P<0.01 vs the NC plus SNL group at the corresponding time points. (i) Effect of microinjection of eIF4G2 siRNA (Si) or negative control siRNA (NC) into the ipsilateral L4 DRG on the expression of p-ERK1 and 2, total ERK1 and 2, and GFAP in the ipsilateral L4 dorsal horn on day 5 after SNL or sham surgery. Left: representative Western immunoblots. Right: statistical summary of densitometric analysis. n=3 mice per group. Two-way anova with repeated measures followed by post hoc Tukey test. Fgroup (1, 8)=30 for p-ERK1, 108 for p-ERK2, 0.50 for ERK1, 0.01 for ERK2, and 128 for GFAP. ∗∗P<0.01 vs the corresponding NC plus sham group. ##P<0.01 vs the corresponding NC plus SNL group. eIF4G2, eukaryotic initiation factor 4 gamma 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GFAP, glial fibrillary acidic protein; p-ERK, phosphorylated extracellular signal-regulated kinase 1; PWF, paw withdrawal frequency; PWL, paw withdrawal latency.