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. 2021 Mar 22;21(5):515. doi: 10.3892/etm.2021.9946

Figure 3.

Figure 3

Effects of droxinostat on cell viability and levels of MMPs and histone acetylation related factors (H4K5ac and H4K8ac) in HAEpi cells. (A) A Cell Counting Kit-8 assay was performed to detect the effect of different concentrations (1, 5, 10 and 20 µM) of droxinostat on HAEpi cell viability following 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. the control group at 48 h; #P<0.05 and ##P<0.01 vs. the control group at 72 h. (B) The effect of droxinostat on the levels of H4K5ac and H4K8ac in HAEpi cells was investigated using western blotting. H4 served as an internal control. The effects of droxinostat on (C) MMP2 and MMP9, and (D) HDAC1, HDAC2 and HDAC6 were detected by ELISA kits. Data are expressed as the mean ± standard deviation from three independent experiments *P<0.05 and **P<0.01 vs. the control group. MMPs, matrix metalloproteinases; H4K, histone H4 lysine; HAEpi, human amniotic epithelial; HDAC, histone deacetylase; ac, acetylated.