Fig. 1.
Dynamic phosphorylation of MpkA in response to micafungin. The control strain was grown in shake flask culture until the mid-exponential growth phase. Micafungin was added at a concentration of 20 ng/ml per 1g/kg DCW and samples (0, 1, 3, 5, 10, 15, 30 min) were removed and immediately frozen. Western blotting was used to quantify the amount of MpkA phosphorylation (A). Fold change band intensities from 2 biological replicates were fit to the kinetic model with Vmax = 1.38 and Kt = 2.01 (B).